 |
|
 |
|
| Author |
Title |
|
| Cambar
J,Barrouillet MP,Delbancut A |
Protective
effects of cytotoxic Cadmium |
|
| Document
Type |
Location |
Language |
| Journal
Article |
Glasgow
Homoeopathic Library |
English |
|
| Source |
Year,
Volume & Pages |
|
| Proc.
52nd LMHI Congr., Seattle, USA |
1997
157-164 |
|
|
| Key
Terms |
TOXINS,
ENVIRONMENTAL POLLUTANTS, MERCURY POISONING |
| Minor
Terms |
HOMEOPATHY,
RESEARCH, METALS,CELLS, POISONING, PREVENTION, ANIMAL |
| Entry
Terms |
Cadmium,experimental
research,mice |
|
| Abstract |
|
The
present study purposed to approach, by a sensitive in vitro
model, renal tubular cell cultures, the mechanism of the protective
effect of very high dilutions of Cd against Cd cytotoxicity.
This protective effect was estimated by different tests as
viability assessed by an automatic procedure using neutral
red, cadmium intracellular uptake or cytoskeleton organization.
|
| We
studied the protective effect of a 5 day pretreatment with succussed
high dilutions (10-20 to 10-40M) of Cd in Cd intoxicated cells.
The control cells were pretreated with succussed dilutions of
culture medium followed by the same metal intoxication as test
cells in the same experimental conditions. |
| The
protective effect of high dilutions is dose- and duration of
the pretreatment dependent. A 5 day pretreatment with 10-30
and 10-40M of Cd induced a very significant decrease in cell
mortality (p<0,05 to p<0,001). For example 3.10-5 M of Cd induced
a mortality of 62,2 +/- 2,15% in the control cells and only
27,67+/- 5,29% in the cells pretreated with 10-40M of Cd (p<0,05).
In order to understand the cellular mechanisms of this protective
effect of high Cd dilutions, we quantified the intracellular
Cd uptake in 5 day pretreated cells with a 10-30M of Cd and
in control cells when both cells were exposed to toxic Cd concentrations
(0,25 mg/l to 2,5 mg/l). Culture medium and intracellular Cd
patterns were assessed by atomic absorption spectrophotometry.
Cd penetration ratio is introduced as the ratio between extracellular
and intracellular Cd. We observed a significant decrease in
Cd uptake ratio in pretreated cells for the lower Cd concentrations.
For example, in control cells, the Cd penetration ratio is 10,40%
and only 6,68% for pretreated cells (<0,05). Immunoflorescence
studies showed a dramatic dose dependant actin an vimentin organisation
change with ponderal Cd concentrations (0,125 to 2. 10-5 M);
but, 10 -40 M Cd pretreated cells appeared less damaged in size
and shape than the control ones. |
| In
conclusion, these data demonstrate that very high dilutions
of heavy metals have a fair protective effect in cells exposed
to cytotoxic concentrations of the same metals after a 5 day
pretreatment. Moreover, this study introduces new sensitive
tools for further investigations to show and understand the
mechanisms of the protective effect of very high dilutions against
nephrotoxic agents in order to reduce their side effects in
clinical use. |
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