Title: Demonstration of Anti–Diabetic Activities of Alloxan in Potentised State– An
Experimental Approach
Objective: To study the
Dynamised and Undynamised preparation of alloxan for its anti-diabetic activity
in albino rats.
Materials
& Methods: Diabetes mellitus was
induced in the albino rats whose blood sugars were within 50-120 mg/dil through
intraperitoneal injections. Three doses of 10-12 mg/g. b. w. at 7 days interval
of alloxan dissolved in distilled water were administered after 12 hours fasting.
Blood sugar estimation was done to confirm the establishment of diabetes
mellitus. The diabetised animals were divided into groups and fed 0.9% saline ,
90% alcohol vehicle, and dynamised/undynamised alloxan 6x, 30x, 200x, and
undynamised vehicle for 30 days and blood sugar estimations were done at 12
hours fasting on first 15 and 30th days Histopathological studies
were also conducted of brain , pituitary gland, pancreases liver, kidney, adrenal glands etc.
Results: Oral administration
of dynamised potencies of alloxan 6x, 30x and 200x at a dose level of 50 µl/100
g.b.w. daily for 30 days, regularly, exhibited slow and steady fall in blood
sugar level when compared to dynamised and undynamised control groups as well
as undynamised alloxan control under identical conditions.
Conclusion: Dynamised and undynamised preparations of Alloxan viz.
6x,30x, and 200x were examined for its anti-diabetic activities in
Alloxan-induced diabetes mellitus albino rats. Oral administration of dynamised
potencies of alloxan 6x, 30, and 200x at a dose level of 50µl/100 gm.b.w daily
for 30 days regularly exhibited slow and steady fall in blood sugar level when
compared to dynamised and undynamised control group as well as undynamised
alloxan under identical conditions. Histopathological and Histomorphometric
studies also raveled that β-cell counts were functional to 30-40 %
populations and protects the β-cell against necrotic effects especially in
dynamised dilutions of alloxan in 30x and 200x potencies. It was also observed
group is more toxic and lethal to animals than dynamised and undynamised
dilutions of alloxan and undynamised alcohol control.
Published in: Drug Research, Homoeopathic Drug Research Institute, Annual Report 1990-91, 23-30.